AirFlow PCR device
This page describes ongoing efforts to build a cheap and reliable PCR machine that combines the flexibility of 3D printed chassis design for air flow heating and cooling with low cost components and customisable control software. The design process is based on incremental trials developing 3D printed isothermal incubators for microtube reactions. The main new challenges are:
Construction of an instrument chassis that tolerate up to 100ºC.
Use of a fan for air circulation that isn't limited by thermal cut-out (most computer fans have a built-in working limit of 70-80ºC as a guard against motor stalling and burnout).
Find a simple way of switching between heating and cooling cycles.
Moving away from XOD as the control software to UiFlow2, which provides a graphical programming interface as well as Micropython compatibility, and integrated support for touchscreen interface.
biomaker.org